Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Limonin attenuates the stemness of breast cancer cells via suppressing MIR216A methylation.

doi: 10.1016/j.biopha.2019.108699

Figure Lengend Snippet: Fig. 3. Limonin decreases the methylation of MIR216A and thus increases miR-216a-3p expression. (A) Represented miRNAs with ectopic expression in MDA- MB-231 cells with or without limonin treatment, shown as heatmap. (B) The levels of represented miRNAs shown in (A) were detected via qRT-PCR assay. (C) A genomic schema of MIR216A gene showing the promoter and the region analyzed for DNA methylation. (D) Genomic DNA from breast cancer cells with limonin treatment or not was collected, and MIR216A promoter CpG methylation status was examined using EpiTech Methyl II PCR primer assay for the MIR216A promoter. (E) Genomic DNA from breast cancer cells and non-tumorigenic cells was collected, and MIR216A promoter CpG methylation status was evaluated. (F) miR-216a-3p level was determined in breast cancer cells with or without Decitabine treatment, and MCF-10A cells with or without GSK J4 HCI treatment. (G) miR-216a-3p level was examined in breast cancer cells with limonin treatment as well as GSK J4 HCI or not. Data were presented as mean ± s.d; **P < 0.01 vs. control.

Article Snippet: Limonin, SKL2001 (Cat # S8320), the activator of Wnt/β-catenin signaling, methyltransferase inhibitor Decitabine (Cat # S1200) and Histone Demethylase inhibitor (GSK J4 HCI, Cat # S7070) were purchased from Selleck.cn (Houston, Texas, USA). pcDNA-WNT3A plasmid was purchased from Addgene (Cat # 35908).

Techniques: Methylation, Expressing, Quantitative RT-PCR, DNA Methylation Assay, CpG Methylation Assay, Control

Journal: Communications Chemistry

Article Title: A Hybrid compound H93 treats prostate cancer by directly binding UHRF1 and promoting protein dimerization

doi: 10.1038/s42004-025-01744-3

Figure Lengend Snippet: a DU145 (Left) or VCaP (Right) cells were treated with indicated concentrations of H93 for 48 h, and the total DNA methylation level was measured by 5-methylcytosine (5mC) DNA dot-blot assays. Experiments were performed in triplicate. b DU145 (Left) or VCaP (Right) cells were treated with 0 (black dots), 10 (black squares) or 20 μM (black triangles) of H93 for 48 h, and the total DNA methylation level was measured by 5mC ELISA assays. The data are represented as the mean ± SD, n = 3 independent experiments. c Quantitated gene expression changes of representative tumor suppressor genes in DU145 (Upper) and VCaP (Lower) cells treated with 0 (empty black circles), 2.5 (black squares), 5 (black triangles), 10 (black rhombus) or 20 μM (black dots) of H93. n = 3 independent experiments. d–f DU145 subcutaneous xenografts were induced in immune-deficient nude mice, and then treated with vehicle (20 ml kg −1 day -1 ) or H93 (50 or 100 mg kg −1 day −1 ) by oral administration. n = 8 per group. The tumor nodes were harvested 28 days after treatment. d The tumor volumes (Left) and weights (Right) were compared with vehicle 20 ml kg −1 day −1 group depicted as black dots, H93 50 mg kg −1 day −1 group as black squares, H93 100 mg kg −1 day −1 group as black triangles. n = 8 animals. e The body weights of nude mice were continuously monitored and vehicle 20 ml kg −1 day −1 group was depicted as black line, H93 50 mg kg −1 day −1 group as red line, H93 100 mg kg −1 day −1 group as glaucous line. n = 8 animals. f Representative images of Hematoxylin-Eosin (HE), Ki67 immunochemistry (IHC) and transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) staining in DU145 xenografts subjected to different concentrations of H93 (Left), and the quantitative data of Ki67 (Middle) and TUNEL (Right) staining, with vehicle 20 ml kg −1 day −1 group depicted as black dots, H93 50 mg kg −1 day −1 group as black squares, H93 100 mg kg −1 day −1 group as black triangles, and n = 40 views. The length of scale bars is 200 μm (×100) or 50 μm (×400). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. The data are shown as mean ± SD.

Article Snippet: Colorimetric TUNEL Apoptosis Assay Kit (Cat#C1098) was purchased from Beyotime Biotech.

Techniques: DNA Methylation Assay, Dot Blot, Enzyme-linked Immunosorbent Assay, Gene Expression, End Labeling, TUNEL Assay, Staining